RNA-Seq analysis in human trophoblast stem cells (CT27) upon depletion of Hippo signaling Component TAZ (WWTR1)

The hippo signaling cofactor TAZ (Also known as WWTR1) regulates expression of genes upon interaction with TEAD transcription factors. Among the TEAD transcription factors, TEAD4 is implicated in regulation of the self-renewal process of human trophoblast stem cells. However, we have a poor understanding about the contribution of TAZ, a co-factor of TEAD4, in that process. Thus, the goal of this study is to define the importance of TAZ in orchestrating global gene expression program in human trophoblast stem cells. Using a RNA interference strategy (RNAi), we depleted TAZ expression in human trophoblast stem cells and compared global gene expression patterns via RNA sequencing (RNA-seq). Three individual experiments were performed. The submitted files contain the data (raw fastq files, count data and deseq2 output) from those experiments. TAZ expression was depleted in CT27 human TSC lines via RNA interference using shRNA. Global gene expressions were monitored by culturing control and TAZ-depleted cells in stem state culture condition.