A serine/arginine-rich splicing factor Srp1 binds to the GUAG consensus and is sumoylated to regulates mRNA splicing in rice blast fungus, Pyricularia oryzae

To reveal how MoSrp1 affects mRNA splicing, we compared the transcriptomes between strains srp1-ko1 and P131, and analyzed defects in mRNA splicing resulted from the loss of MoSRP1. Two-day-old vegetative hyphae of wild-type strain P131 and the MoSRP1 mutant srp1-ko1 shaken in liquid CM were harvested and transcript abundance analysed using RNA-seq, two biological replicates per sample.To determine the binding motif of MoSrp1, two strains, Srp1-flag-3 and Srp1-flag-7, expressing the MoSrp1-3Flag fusion were generated, two-day-old vegetative hyphae of strains shaken in liquid CM were harvested, and were used for the RNA immunoprecipitation and sequencing (RIP-seq).