AIMS Shambley Creek Dissolved Organic Carbon Data (AIMS_SE_WHR_DOCS)

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr. Between 14 October 2021 and 7 July 2024, grab samples for Dissolved Organic Carbon concentration (DOC, mg/L) collected at the watershed outlet (WHM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2). DOC samples were filtered through pre-ashed, 0.7-micron glass fiber filters (Whatman GF/F) into acid-washed, 60-mL amber high-density polyethylene (HDPE) bottles. DOC samples were preserved by acidifying to a ph = 2 using concentrated HCl and refrigerated until analysis within 28 days of collection. DOC concentrations were determined as non-purgeable organic carbon (NPOC) using a total organic carbon analyzer (Schimadzu TOC-V).