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Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28

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https://zenodo.org/record/2598803
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Project: Biophysical investigation of purified HTT protein samples Experiment: Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells Date completed:­ 2019/01/28 Rationale: HTT lacking the unstructured region of exon1 (aa. 1-78 which are not resolved in the cryoEM model PDB ID 6EZ8) may serve as a useful control when determining exon1-dependent functionality of the HTT protein. Apo and HAP40-bound HTT79-3144 will be produced from Sf9 insect cell expression system.
创建时间:
2024-07-24
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