Fold change in gene expression after inoculation with entry-defective HSV-1.

HFFs were stimulated with 1000 particles/cell of ΔgB, ΔgD or ΔgH HSV-1 for six hours and a cDNA microarray corresponding to targets of 19 signalling pathways was used to detect changes in cellular gene expression when compared to mock-infected. Infections and mock infections were carried out in duplicate. A number of signalling pathways were shown to be stimulated by one or more of the entry-defective mutants. These data were used to independently verify changes in expression using real-time PCR.