Next Generation Sequencing Facilitates Quantitative Analysis of THP-1 cells under different stimulas.

To explore the role of human RNase1 on macrophage polarization, we induced human monocytes (THP-1) to differentiate into uncommitted macrophages and then polarized them to different phenotypes by lipopolysaccharide (LPS)/interferon (IFN)-?, interleukin (IL)-4, or RNase1 as a stimulus. We then performed gene expression profiling analysis using data obtained from RNA-seq of Nivolumab reponders and non-reponsers. Overall design: Comparative gene expression profiling analysis of RNA-seq data for THP-1 cells under INF-? plus LPS, IL-4 or RNase1 treatment.