Synergistic effects of 5-Fluorouracil in combination with Salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in Colorectal Cancer

5-FU resistance is considered to be a possible reason for the failure of conventional drug treatment of colorectal cancer (CRC). Recently, salinomycin (SAL), as a selective inhibitor of cancer stem cells (CSCs), has been used to sensitize and attenuate a variety of solid tumor chemotherapy drugs. In our study, our goal was to combine SAL with 5-FU to explore whether increase the sensitivity of CRC to 5-FU and the molecular mechanism that involved in enhancing 5-FU sensitivity and promoting tumor cell chemotherapeutic death. SW480 cell lines were treated with 0.1% DMSO, 2.5uM Salinomycin, 5uM 5-Fluorouracil, 2.5uM Sal and 5uM 5-FU for 20h and then RNA-seq was performed.