Transcription of HIV-1 gag in the spermatozoa transfected with unmethylated and methylated plasmids, respectively, and in the sperm-derived 2-cell embryos.*

*By using real-time quantitative RT-PCR and 2−△△Ct method, the data were presented as the fold change in gene expression normalized to an endogenous reference gene and relative to HIV-1 gag in the spermatozoa transfected with the methylated plasmid, and in the sperm-derived 2-cell embryos.