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Fosmid clone with cellulose degrading gene prepared from metagenomic DNA of degrading wood samples from Sambhar Lake

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NIAID Data Ecosystem2026-04-25 收录
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To identify novel endoglucanases and beta glucosidase gene(s) from extreme halophiles of Sambhar Lake in Rajasthan a fosmid library of ~40000 clones was prepared. Selected positive clones were sequenced either by sub-cloning (EG14) followed by end sequencing using primer walking approach (EG14); or direct sequencing of fosmid DNA (EG3, EG35, EG78, and BG1) on NGS platform (ION S5).

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2020-09-04
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