Evolutionary stability inferred for a free ranging lizard with sex-reversal

The sex of vertebrates is typically determined genetically, but reptile sex can also be determined by incubation temperature. In some reptiles, temperature interacts with chromosomal genotype to reverse sex, potentially leading to transitions from a chromosomal to a temperature-dependent sex determining system. The Central Bearded Dragon, Pogona vitticeps, exhibits female heterogamety (ZZ/ZW) but can have its sex reversed from ZZ male to ZZ female by high incubation temperatures. The species exhibits sex-reversal in the wild and it has been suggested that climate change could be increasing the frequency of reversal. Transitions to temperature-dependent sex determination require low levels of gene flow and high (> 50%) rates of sex-reversal but would have increased likelihood if sex-reversed individuals have higher survival and greater reproduction compared with concordant ZW females. Here, we combine genotype-by-sequencing, identification of phenotypic and chromosomal sex, exhaustive..., , , # Evolutionary stability inferred for a free ranging lizard with sex-reversal

Data description:

Molecular dataset: final.gl.drop
A total of 207 lizards were captured during sampling trips across south west queensland and once individuals were genotyped for their chromosomal sex this amounted to: ZZf (n = 18), ZWf (n = 98), ZZm (n = 91). Reduced representation sequencing (Kilian et al., 2012) was used to genotype individuals in order to determine the levels of population genetic structure (as an indicator of gene flow) across the study area. Genomic DNA from tail tissue and blood samples was extracted using two DNA extraction methods appropriate for each sample type: (a) Qiagen's Gentra® Puregene® DNA purification kit for tail tips, or (b) Whatman Elute quick extraction protocol for blood on WhatmanTM FTATM Elute Cards. Specifically, DNA was extracted and sequenced and informative SNP markers identified using the commercial provider Diversity Arrays Technology (DArT Pty Ltd, Canberra, ...