Single cell transcriptomic studies of an Antigen-Specific Dual Microparticle Treatment of a Mouse Model of Multiple Sclerosis

A microparticle system using GMCSF, TGF-b, and VD3 along with disease specific antigen (Myelin Oligodendrocyte Protein) shows effiacy in treating a mouse model of multiple sclerosis, while GMCSF, TGF-b, and VD3 and irrelevant OVA antigen did not reduce scores. Here we show that pro-inflammatory and cell cycle genes are downregulated in multiple populations. scRNA-seq further revealed a reduction in antigen-presentation gene signature. Overall design: One pair of mice was from the spinal cord and two mice from the ingunial lymph nodes. Mice treated with an antigen-specific microparticle system were compared to mice treated with an irrelevant antigen. Results compared OVA and MOG in the CNS and OVA vs. MOG in the lymph node separately.