Real time PCR quantification. Threshold cycle for fpr1ver3 and fpr2ver3 transcripts in untreated Ver3 cells, or after challenges with either 0.5 mM MV, 1 mM H2O2 or 900 J m-2 UV. controls were the exp
Sequences of primers and probes used for Real-Time PCR. Sequences related to gene type X collagen and MMP13 are proprietary to Applied Biosystems Inc. and not disclosed.
Calculations of delta Ct values for the five Spiroplasma RIP genes. Results for each strain (Uganda or Brazil) are reported in separate worksheets. In addition one worksheet contains the summarized re