TS alleles of gyrase and Topo IV decrease diffusible chromosome supercoiling at Cs 85.
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Resolution assays for a Lac-Gn module introduced at Cs 85 were measured in WT and TS mutations of gyrase and Topo IV. Two mutations in gyrA and gyrB genes plus 1 TS allele of the parC and parE genes of Topo IV were tested in exponential phase at 30°. The apparent σD was estimated from the graph in Figure 1C. The MIF is calculated as resolution efficiency of the WT divided by the mutant and it indicates the magnitude effects of each allele on recombination at Cs 85.
创建时间：
2015-12-02
