Vitis vinifera Transcriptome or Gene expression. Vitis vinifera

This is a time course experiment for inflorescence development in latent buds sampled from green canes at a single time point. Keywords: Floral Development Overall design: The 12 latent buds and the apical shoot meristem were sampled. The expression profile for each bud position was analyzed to determine gene expression patterns linked to the timing of gene expression for inflorescence development. Bud #1 (actually 2nd bud on a cane as the first one was not collected) is the oldest and will express genes involved in later developmental processes. Bud #12 is the youngest and will express genes that are responsible for the earlier processes in inflorescence development. The apical shoot meristem is a control as it does not develop an inflorescence. Biotin labeled probes were prepared from the extracted RNA from pooled green latent buds and apical shoot meristem and hybridized to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridization station was randomized using the AlgDesign package from R.