CRAC of yeast RNA polymerase II and RNases H in asynchronous, G1-arrested, and S-phase cells.

We obtained RNA polymerase II and RNases H occupancy profiles across the genome of S.cerevisiae strains: Sen1 depleted cells using the degron system (including no addition of IAA); sen1-3, RNases H deleted, and wild-type cells. This allowed us to compare RNAPII and RNases H occupancy profiles in different genetic context and/or phases of the cell cycle.我们通过降解标签系统（degron system），获取了酿酒酵母（S.cerevisiae）不同菌株的全基因组范围内RNA聚合酶II（RNA polymerase II）与核糖核酸酶H（RNases H）的结合占据图谱，所涉菌株包括：采用该系统构建的Sen1耗竭细胞（含未添加IAA的组别）、sen1-3突变株、核糖核酸酶H敲除株以及野生型细胞。本数据集使我们得以在不同遗传背景和/或细胞周期时相下，对比RNA聚合酶II与核糖核酸酶H的结合占据图谱。