K27me3 deposition by FIE and EMF2 in ap1 cal AP1-GR

The termination of floral meristems is regulated by the MADS domain transcription factor AGAMOUS (AG) by preventing the maintenance of the H3K27me3 mark along the KNUCKLES (KNU) coding sequence. After 2 days of cell divisions, KNU transcription is activated prior to floral meristem termination. However, how many other downstream genes are temporally regulated by this intrinsic epigenetic timer and what kind of function those genes have are not known yet. Here, we identify AG direct targets regulated through cell cycle-coupled H3K27me3 dilution after removal of Polycomb repressive in a genome-wide manner using floral induction system (ap1 cal AP1-GR).