The NSL complex is required for telomere maintenance and primary piRNA biogenesis from telomeric clusters

Telomeres cap and protect chromosome ends. Drosophila telomeres consist of repetitive sequences dominated by retrotransposons. Telomeric sequences are transcribed and participate in a negative feedback loop in which they are processed into self-targeting piRNA on the one hand and are serve as the sole source of the transposase responsible for telomere maintenance on the other hand. We show that the tight regulation of the expression of telomeric sequences in the germline is regulated by the NSL complex. As a consequence, both primary biogenesis from telomeric piRNA clusters as well as telomere stability are impaired in cells following NSL complex depletion. The study characterizes the role of a transcriptional regulatory complex in the germline of D. melanogaster in regulating the piRNA pathway. Please note that [1] the input samples do not have a corresponding processed file because the processed file is the INPUT-Normalized bigwig file. [2] The diffexpr-resultsNSL*vsWhite.csv cwere generated from the whiteRNAi_*ovary_RNAseq and NSL*RNAi_ovary_RNAseq samples and is linked to the corresponding NSL*RNAi_ovary_RNAseq sample records.