Three-dimensional chromatin architecture in DRG neurons following sciatic nerve injury in WT and Rad21 KO mice.

Axonal regeneration after injury requires active gene transcription. Whether chromatin 3D genome architecture is required for the neuronal regenerative transcriptional programme is unexplored. Here we addressed this question in a model of sciatic nerve injury by combinatorial Hi-C, promoter-capture Hi-C, CUT&Tag for H3K27ac and RNA-seq in wild-type and cohesin-deficient sensory dorsal root ganglia neurons. Neuronal nuclei were isolated from mouse sciatic dorsal root ganglia 3 days after sciatic nerve crush (SNC) or sham or in naïve conditions from WT (INTACT-AdvillinCre) and Rad21 KO (INTACT-Scc1flox/flox-AdvillinCre) mice, 14 days after tamoxifen injection. Neuronal nuclei were then used for RNA-seq (N=3 replicates), Hi-C (N=3 or 2 replicates), promoter Hi-C (N=3 replicates), and H3K27ac Cut&Tag (N=3 replicates).