Hyaluronic acid-engineered Bcl-2 inhibitor nanocrystals for site-specific delivery to breast tumor cells - supplementary table and figure

<b>Aim: </b>This investigation aims to repurpose venetoclax using hyaluronic acid-coated venetoclax nanocrystals (HA-VEN-NCs) to target breast cancer. <b>Materials &amp; methods: </b>An antisolvent precipitation method was used to fabricate the nanocrystals and optimize them using central composite design. Hyaluronic acid (HA)-coated and -uncoated nanocrystals were compared in terms of <i>in vitro </i>drug release, cell line studies, CD44-expressing breast tumor cell binding capability and anticancer activity. <b>Results: </b>HA-VEN-NCs and venetoclax nanocrystals (VEN-NCs) showed pH-responsive drug-release behavior, exhibiting sustained release at pH 6.8. Our extensive <i>in vitro </i>cell line investigation showed that HA-VEN-NCs efficiently bind to CD44-expressing breast tumor cells and possess excellent anticancer activity (half maximal inhibitory concentration: 2.00 μg<b>/</b>ml) compared with VEN-NCs. <b>Conclusion: </b>Our findings anticipate that HA-VENNCs could serve as valuable nanoplatforms for cancer treatments in the future.