The transcriptional alteration and non-invasive biomarker potential of peripheral blood microRNAs in patients with early-onset pre-eclampsia

Early-onset preeclampsia (EOPE) is a severe pregnancy complication associated with significant maternal and fetal morbidity and mortality. Currently, there is a critical need for accurate, non-invasive biomarkers to facilitate early diagnosis and effective management of EOPE. In this study, we aimed to investigate the transcriptional alterations and non-invasive biomarker potential of peripheral blood microRNAs in patients with EOPE. Through our research, we successfully identified two reliable plasma miRNA biomarkers and proposed a circulating two-miRNA panel for the non-invasive early detection of EOPE. Additionally, we independently validated our findings in different patient cohorts using various technological platforms. Overall design: To investigate non-invasive biomarker potential of peripheral blood microRNAs in early-onset pre-eclampsia, we conducted a two-phase discovery and validation study. We then performed miRNA expression profile analysis using data from RNA-seq of 17 plasma samples (5 EOPE, 5 LOPE, and 7 non-disease controls) to identify potential miRNA biomarkers and validate them in external independent cohorts.