Transcriptional control of thermogenesis by early B cell proteins [ChIP-seq]

Early B Cell Factor 2 (EBF2) is required for brown adipose tissue basal thermogenic gene expression; however, it was unknown whether chronic cold exposure normalized the enhancer landscape of Ebf2 mutant mice. We profiled H3K27Ac binding in Ebf2 WT and mutant brown adipose tissue in mice housed at room temperature or one week of cold exposure. Overall design: H3K27Ac ChIP-Seq was performed in control and Ebf2 mutant brown adipose tissue from adult mice acclimated to room temperature or 1 week of cold exposure.