Expression profiling of the aging epidermis [RNA seq]

Whole transcriptome sequencing dta generated from epidermal samples (suction blister roofs) of healthy female subjects between 21 and 76 years. Aim of the project was the investigation of non-linearities in the human aging progression using an integrative multi-omics analysis. RNA was extracted from suction blisters taken from the volar forearms of each subject, cDNA converted and then sequenced on an Illumina HiSeq 2500 (single-end, 50 bp) to an approximate sequencing depth of 100 million reads per sample.