Data for: Testing the population-level effects of stress-induced susceptibility in the ranavirus-wood frog system

In this study we tracked experimentally-induced epidemics of ranavirus infections in 96 populations of wood frog (Lithobates sylvaticus) larvae (in addition to 32 control populations) raised in mesocosms with low or high salinity (well water, 109-177uS/cm; or well water + road salt 1900-2000uS/cm) crossed factorially with ambient or elevated (ambient + 3°C) water temperature. Epidemics were initiated by the introduction of two ranavirus-infected larvae (waterbath exposed to 2.5 × 104 plaque-forming units/mL of a local ranavirus isolate for the prior 24h) into each of the 96 virus mesocosms or two uninfected larvae in the 32 control mesocosms. Mesocosms were checked daily for carcasses and metamorphosing individuals (=front limb emergence), which were removed and frozen for later analyses. Recovered carcasses and a subset of metamorphosing individuals were then screened for ranavirus infection with a quantitative realtime PCR assay. 

Methods
This data set consists of the identity (SampleID, mesocosm [Tank]), treatment information, date of collection (Mortality_Date or date of metamorphosis [Meta_Date]), and infection status (PCR positive or negative [Pos], mean ranavirus gene copy number [MeanCopy] if positive) for each individual animal recovered during the daily checks of the experimentally-induced ranavirus epidemics. More details are available in the README file