RNA-seq analysis of Lrig1+ and Lrig1- CD4+ T cells in mouse spleen

Regulatory T cell (Treg) is the immune-suppressive T cell subset and the crucial component for preventing autoimmunity. The molecules defining the suppressive population of T cells phenotypically and functionally remain to be discovered. In this study, we report that Lrig1 is highly expressed in suppressive CD4+ T cell populations. We performed RNA-seq analysis of CD4+Lrig1+ and CD4+Lrig1- T cells from mouse splenocytes to analyze the genes involeved in the suppressive function of T cells. Overall design: To investigate the transcriptional difference between Lrig1+ and Lrig1- CD4+ T cells, we isolated Lrig1+ and Lrig1- CD4+ T cells from spleen of 7 weeks of age female C57BL/6 mice using flow cytometry.