Composition of the Diets.

aMineral mixture (g/kg mixture):Calcium carbonate, 357; Potassium Phosphate, 196; Potassium Citrate, 70.78; Sodium Chloride, 78; Potassium Sulphate, 46.6; Magnesium Oxide, 24; Ferric Citrate, 6.06; Zinc Carbonate, 1.65; Manganous Carbonate, 0.63; Cupric Carbonate, 0.3; Potassium Iodate, 0.01; Sodium Selenate, 0.01; Ammonium Paramolybdate, 0.007; Sodium Metasilicate, 1.45; Chromium Potassium Sulphate, 0.275; Lithium Chloride, 0.01; Boric Acid, 0.08; Sodium Fluoride, 0.06; Nickel Carbonate, 0.03; Ammonium Vanadate, 0.006; Sucrose, 221.02.
bVitamin mixture (g/kg mixture):Nicotinic Acid, 3; Calcium Pantothenate, 1.6; Pyridoxine-HCl, 0.7; Thiamin HCl, 0.6; Riboflavin, 0.6; D-Biotin, 0.02; Vitamin B12 (in 0.1% Mannitol), 2.5;Vitamin E, 15;Vitamin A, 0.8; Vitamin D-3, 0.25;Vitamin K, 0.075; Folic acid, 0.2 (control) and Sucrose 974.655, was used to make total weight of the vitamin mixture to 1 kg.
Control- Normal folic acid, normal vitamin B12; BD- vitamin B12 deficient; FD- folic acid deficient; BDO- vitamin B12 deficient and omega-3 fatty acid supplemented; FDO- folic acid deficient and omega-3 fatty acid supplemented.