X-ray diffraction data used for the determination of the crystal structure of the tungsten-dependent Aldehyde:ferredoxin oxidoreductase from Clostridium autoethanogenum

Crystallisation and Data collection Crystallisation was performed anaerobically by initial screening at 20 °C using the sitting drop method on 96-Well MRC 2-Drop polystyrene Crystallisation Plates (SWISSCI) in a Coy tent containing an N2/H2 (97:3%) atmosphere. The reservoir chamber was filled with 90 μl of crystallisation condition (SG1TM crystallisation screen from Molecular Dimensions), and the crystallisation drop was formed by spotting 0.55 μl of purified protein with 0.55 μl of precipitant. The protein was crystallised at 8.6 mg.ml-1 in storage buffer, and the precipitant solution contained 200 mM MgCl2 x 6 H2O, 100 mM Bis-Tris pH 6.5, 25% (w/v) PEG 3,350. The crystals were soaked in the crystallisation solution supplemented with 25% (v/v) glycerol for a few seconds before freezing in liquid nitrogen. The diffraction experiments used for the deposited model were performed at 100 K on the beamline PROXIMA-1 from SOLEIL. The data were collected at 1.21434 Å.