Data from: Preparation of isolated guard cells, containing cell walls, from Vicia faba

Stomatal movement, initiated by specialized epidermal cells known as guard
cells (GCs), plays a pivotal role in plant gas exchange and water use
efficiency. Despite protocols existing for isolating GCs through
proplasting for carrying out biochemical, physiological, and molecular
studies, protocols for isolating GCs with their cell walls still intact
have been lacking in the literature. In this paper, we introduce a method
for the isolation of complete GCs from Vicia faba and show their membrane
to remain impermeable through propidium iodide staining. This methodology
enables further in-depth analyses into the cell wall composition of GCs,
facilitating our understanding of structure-function relationship
governing reversible actuation within cells.