A Homology Independent Sequence Replacement Strategy in Human Cells using CRISPR/Cas9

Here we demonstrate the ability to create two Cas9 driven breaks in mammalian cells and replace the intervening sequence with an introduced donor sequence; we name this method Replace (Rational enjoining protocol delivering a targeted sequence exchange). Here we demonstrate removal of an exon in endogenous human genes and its replacement with a spliced reporter element showing the potential for exon replacement for therapeutic or research applications. This is the deep sequencing data from the study focusing on a synthetic reproter system in HeLa cells and in POLB exon 5 of K562 cells.