Study on the related targets and molecular mechanism of Xiaojianzhong decoction and gastric cancer.docx

This study aims to deeply explore the potential molecular mechanisms of Xiaojianzhong Decoction in the treatment of gastric cancer by means of network pharmacology and molecular docking techniques, combined with bioinformatics analysis methods. The RNAseq data of gastric cancer were obtained from the UCSC Xena database, and the differentially expressed genes between gastric cancer tissues and normal tissues were screened out. The main components and their targets of Xiaojianzhong Decoction were obtained from the BATMAN-TCM 2.0 database, and then the associated targets between Xiaojianzhong Decoction and gastric cancer were screened out. The protein-protein interaction (PPI) network was constructed with the help of the STRING database, and the key targets were screened out for functional enrichment analysis. Finally, molecular docking technology was used to verify the binding activity between the key targets and the components of Xiaojianzhong Decoction. The research results showed that a total of 2,544 significantly upregulated genes and 420 significantly downregulated genes were screened out, and there were 250 associated targets between Xiaojianzhong Decoction and gastric cancer. The PPI network analysis indicated that CCND1, CXCR4, and FN1 were the core targets. The functional enrichment analysis pointed out that these targets were mainly involved in pathways such as the cell cycle, inflammatory response, and immune regulation. The molecular docking results showed that the main components of Xiaojianzhong Decoction had strong binding activity with the core targets. In conclusion, Xiaojianzhong Decoction may affect the cell cycle, inflammatory response, and immune microenvironment by regulating targets such as CCND1, CXCR4, and FN1, thereby exerting an anti-gastric cancer effect.