RNA-seq of Helicobacter pylori N6 wild-type and HP1021 deletion mutant under microaerophilic and aerobic conditions (optimal growth and oxidative stress conditions, respectively)

We performed transcriptome (RNA-seq) analyses for Helicobacter pylori N6 wild-type (WT) and HP1021 deletion mutant (?HP1021::aphA-3) under oxidative stress and optimal microaerobic growth conditions. The expression of 411 genes was affected by oxidative stress in stressed wild-type cells (WTS) compared to non-stressed cells (WT). Interestingly, ?HP1021 did not respond to oxidative stress. A comparison of genes expressed in the ?HP1021 and WT strains under optimal growth conditions revealed 191 differently expressed genes. Moreover, transcriptional changes and overall final protein levels correlated across multiple genes. The data were validated through RT-qPCR and phenotype experiments for selected processes.