G3bp1-miR-1 axis regulates cardiomyocyte hypertrophy

We examine the role of G3bp1, a RNA binding protein and site specific endoribonuclease in gene expression in isolated neonatal cardiomyocytes. RNAseq data from cardiomyocytes were infected with adenoviruses expressing shRNA against G3bp1 (ad-siG3bp1) or Luciferase (ad-siLUC, control) showed significant decrease in transcript abudnnace of cardiac-enriched genes involved in Calcium handling, contraction, action potential and sacromere function. On the other hand increase was observed in genes that regulate Hippo, TNF and TGFb signaling. Knockdown of G3bp1 inhibited endothelin-1 induced cardiomyocyte hypertrophy. Neonatal Rat Ventricular Mycoytes (NRVM) were isolated and cultured. NRVMs from 3 independent cultures were infected with ad-siG3bp1 or ad-siLUC (control) for 48hrs, the knockdown of endogenous G3bp1 validated and the samples were sent for RNAseq to Active Motif