Detection of flow-sorted <i>C. parvum</i> oocysts using qPCR¹.

1Results presented are the average ± standard deviation of four independent experiments. QPCR was performed in triplicate reactions for each experiment. Flow-sorted oocysts spiked in reagent water were used to determine detection limits of the different qPCR assays evaluated. 2CT values ± standard deviation. Numbers in parentheses indicate the number of positive samples (CT values <40) over the total reactions performed. 3“Mock” flow-sorted sample containing no oocysts. *, Not detected (CT >40).