Multi-scale structure of chromatin condensates explains phase separation and material properties

The structure and interaction networks of molecules within biomolecular condensates are poorly understood. Using cryo-electron tomography and molecular dynamics simulations, we elucidated the structure of phase separated chromatin condensates across scales, from individual amino acids to network architecture. We found that internucleosomal DNA linker length controls nucleosome arrangement and histone tail interactions, shaping the structure of individual chromatin molecules within and outside condensates. This structural modulation determines the balance between intra- and intermolecular interactions, which governs the molecular network, thermodynamic stability, and material properties of chromatin condensates. Mammalian nuclei contain dense clusters of nucleosomes whose non-random organization is mirrored by the reconstituted condensates. Our work explains how the structure of individual chromatin molecules determines physical properties of chromatin condensates and cellular chromatin ..., , , # Multi-scale structure of chromatin condensates rationalizes phase separation and material properties

Dataset DOI: 10.5061/dryad.p2ngf1w45

## Description of the data and file structure

This repo is for the data and its associated analysis that used in the multi-scale chromatin structure paper! The data includes:

`tomograms_fiber_tracing_25bp_condensates.tar.gz`, which contains tomograms and star files for the traced nucleosome arrays that can be open using ChimeraX

`tomograms_fiber_tracing_25bp_dilute.tar.gz`, similar to previous one, but in 25bp chromatin in low salt dilute phase

`tomograms_fiber_tracing_30bp_condensates.tar.gz`, similar to previous one, but in 30bp condensate

`tomograms_fiber_tracing_30bp_dilute.tar.gz`, similar to previous one, but in 30bp chromatin in low salt dilute phase

Description:  tomograms from 30bp chromatin and star files for the traced nucleosome arrays that can be open using ChimeraX

**Parameters**
ChimeraX automatically reads the pixel size fro...