RNAseq of Bbs1 KO Zebrafish larval eyes

Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal dystrophy, intellectual disability, polydactyly, obesity and renal anomalies. In photoreceptors (PR), light sensation occurs in outer segments (OSs), which are specialized primary cilia. BBS1, the major BBS gene, is part of a protein complex called BBSome, which is involved in intracellular protein transport. However, the precise function of BBS1/BBSome in controlling ciliary content in PRs remains unclear. To investigate the transcriptional consequences of a dysfunctional BBSome we established a new bbs1 zebrafish mutant. We conducted an eye specific RNAseq experiment using 5 and 10 day old maternal zygotic mutants and their heterozygous (unaffected) siblings as control. Differential expression analysis was used to investigate the expression levels at 5 and 10 days.