CITE-seq analysis of mouse LSK cells
收藏NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP249859
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资源简介：
We performed cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) of the LSK compartment of TdT-reporter and TdT-fate mapping mice, in order to elucidate the heterogeneity and developmental trajectories within the MPP (lymphoid-primed multipotent progenitor) and HSC (hematopoietic stem cell) compartments. Overall design: Lineage-Sca1+cKit+ (LSK) cells from 4 TdT[hCD4]-TdT[iCre]-Rosa26[YFP] mice were sorted, stained with antibodies coupled to oligonucleotides directed against hCD4, Flt3, CD48, CD150, CD9, CD41, CD55, CD105, CD115, CXCR4, and ESAM, and loaded on separate wells of a 10X Genomics Chromium Single Cell Controller.
创建时间：
2023-08-31
