RNA-seq for Melatonin treated mice after traumatic brain injury

Nine C57BL/6J male mice (6-8 weeks) were randomly divided into three groups, sham, traumatic brain injury (TBI), and melatonin group (n = 3). The sham group only received craniectomy, while TBI and the melatonin group received a controlled cortical impact (CCI) after craniectomy. The melatonin treatment group received an intraperitoneal injection of 2 mg/kg melatonin per day for 3 consecutive days (including impact day) after TBI. On the third day after TBI, all mice were sacrificed by cervical dislocation, and the brain tissues of the injured hemicerebrum were taken following storage in liquid nitrogen and sent to BGI for the second-generation transcriptome sequencing.

搜集汇总

背景与挑战

背景概述

该数据集包含9只雄性C57BL/6J小鼠的RNA-seq数据，研究褪黑素对创伤性脑损伤后基因表达的影响。小鼠分为假手术组、TBI组和褪黑素治疗组（每组3只），褪黑素组在TBI后连续3天腹腔注射2mg/kg褪黑素，第3天取损伤半脑组织进行二代转录组测序。