Expression analysis of miR-302 knockout and wild-type embryos during neurulation

Wild-type or miR-302 knockout tissue was isolated from embryos during neurulation in replicate Performed in biological triplicate except for E7.5 WT which was in duplicate and E9.5 time points which were in quadruplicate. Biological replicate samples represent independent embryos. Total RNA collected with Trizol. All processing conducted at the UCLA Neuroscience Genomics Core. MouseRef-8 v2.0 Expression BeadChips.野生型（Wild-type）或miR-302敲除（miR-302 knockout）的组织于神经胚形成（neurulation）阶段从胚胎中分离。本实验采用三次生物学重复设置，其中E7.5野生型样本仅设置两次生物学重复，E9.5时间点样本设置四次生物学重复。生物学重复样本均来自独立胚胎。总RNA通过Trizol试剂提取。所有实验处理均在加州大学洛杉矶分校（University of California, Los Angeles, UCLA）神经基因组学核心实验室完成。检测采用MouseRef-8 v2.0表达微珠芯片（Expression BeadChips）。