NEON Biorepository Fish Collection (Vouchers)

This collection contains fish vouchers collected from wadeable streams and lakes (NEON sample class: fsh_perFish_in.voucherSampleID). Fish are sampled twice per year at lakes and wadeable stream sites, during spring and fall. Ten sampling reaches or segments are established at each site; with 3 fixed reaches sampled during every sampling bout and a random subset of 3 additional reaches or segments selected for sampling each year. Fish are sampled using a combination of electrofishing, gill-nets and mini-fyke nets. Field technicians identify fish to the lowest practical taxonomic level and then weigh and measure a subset of captured individuals before releasing. Only mortalities and individuals that require euthanasia due to injuries are vouchered. Vouchers that are in good condition are fixed in 10% buffered formalin preservative and then transferred to 70% ethanol or isopropanol for long-term storage. Vouchers that are in poor condition are not fixed but preserved in 95% ethanol. Specimens are archived in a temperature (17-18°C) and humidity controlled environment. See related links below for protocols and NEON related data products.本数据集收录了从可涉水溪流与湖泊中采集的鱼类标本（NEON样本类别：fsh_perFish_in.voucherSampleID）。在湖泊与可涉水溪流样点，每年春季与秋季各开展一次鱼类采样。每个样点设置10个采样断面，其中3个固定断面在每次采样时均实施采样，另外每年随机选取3个补充断面开展采样。鱼类采样采用电捕鱼法（electrofishing）、刺网（gill-nets）与迷你渔篓网（mini-fyke nets）相结合的方式。野外技术人员将鱼类鉴定至实际可行的最低分类阶元，随后对部分捕获个体进行称重与测量，之后将其放归自然。仅对死亡个体以及因受伤需实施安乐死的个体制作标本留存。状态完好的标本先以10%缓冲福尔马林固定，随后转移至70%乙醇或异丙醇中进行长期保存；状态较差的标本无需固定，直接以95%乙醇保存。所有标本均存档于温湿度可控的环境中，温度控制在17-18℃。相关实验方案与NEON相关数据产品请参阅下方链接。