pig gut metagenome Metagenome

In this study, we evaluated age-related alterations in feces of suckling and newly weaned piglets receiving sow milk or additionally creep feed from day of life (DoL) 10. Additionally, we compared the composition of the bacterial community in feces of different colors (brown, dark brown, yellow and grey) and consistencies (balls, soft and very soft) as well as from different sample types (feces versus swab samples). Feces were collected at DoL 2, 6, 13, 20, 27, 30 and 34 (n=5/diet/sex/day). The DNA was extracted from gastric and caecal digesta which was followed by 16S rRNA gene using Illumina chemistry and platform and a paired end approach. The V3-V4 hypervariable regions of bacterial 16S rRNA gene were amplified using a 250 bp read length paired-end protocol (Microsynth, Balgach, Switzerland) on an Illumina MiSeq sequencing v2 platform, including the 16S rRNA, library preparation and sequencing.