Single cell analysis of innate lymphoid cells and T lymphocytes in the small intestinal resection of Crohn’s disease patient versus those of adjacent non-inflamed tissue.

The focus of this study is to define the full spectrum of T cells and innate lymphocytes in the context in human inflammatory bowel disease. Results from this study may yield novel findings on the heterogeneity of these lymphocytes and determine how they change in the inflamed human intestine. CD45+, CD19/CD14/CD123/ Fc-epsilon-RI-alpha/CD34/CD94-, CD4-, CD127+ILCs (7,000) and CD45+CD3+ T cells (28,000) were sorted from the resection of inflamed Crohn’s disease patient versus adjacent tissue.