Co-evolving JAK2V617F+ relapsed AML and donor T cells with PD-1 blockade after stem cell transplantation: an index case [BCR-seq]

JAK2V617+ myeloproliferative disease has been reported to overexpress PD-L1 rationalizing therapeutic PD-1 blockade. We performed an integrated single cell analysis on samples from an exceptional responder to nivolumab to elucidate determinants of response in this patient. Mononuclear cells from peripheral blood were obtained from one patient with transient response to nivolumab and sequenced using 5' scRNA-seq/CITE-seq/scTCR-seq/scBCR-seq on the 10x platform. Gene expression (scRNA-seq) and antibody capture (CITE-seq) are contained in the same feature matrix. scTCR-seq and scBCR-seq are provided individually. Raw reads of gene expression are provided as bam files and raw reads of CITE-seq, scTCR-seq and scBCR-seq as fastq files.