Genome-wide maps of TAF2 (ChIP-seq) and Gene expression profiles upon TAF2 depletion (RNA-seq)

TAF2 is the second largest subunit of general transcription factor TFIID. Although TFIID complex is putatively considered as a general initiation factor, gene-specific functions have been reported for several TAF subunits within it. To gain insights for TAF2 function in transcriptional regulation, genomic occupancy of TAF2 and transcriptiome of cells depleted of TAF2 are examined. Genome-wide binding/occupancy profiling of TAF2, TBP, and histone marks by ChIP-seq in HCT116 cells. Transcriptome profiling by RNA-seq in control and shTAF2-treated HCT116 cells .