Intratracheal aerosolization of viral vectors to newborn pig airways - Supplemental Protocol and Methods

<b>Supplementary
Protocol. </b> Intratracheal
aerosolization of viral vectors to newborn pig airways.

Supplemental Protocol
and Methods for stepwise protocol.

<b>Article Abstract: </b> Gene
therapy for airway diseases requires efficient delivery of nucleic acids to the
airways. In small animal models, gene delivery reagents are commonly delivered
as a bolus dose. However, large animal models are often more relevant for transition
from preclinical studies to human trials. Aerosolizing viral vectors to the
lungs of large animals can maximize anatomical distribution. Here we describe a
technique for aerosolization of viral vectors to the airways of newborn pigs.
Briefly, a pig is anesthetized, intubated with an endotracheal tube, and a
microsprayer is passed through the endotracheal tube. A fine mist is then
sprayed into the distal trachea. Widespread and uniform distribution of
transgene expression is critical for developing successful lung gene therapy
treatments.