Transcriptomic Comparison of Common Nontyphoidal Salmonella Serovars Provides Greater Resolution for Understanding Differences in Virulence. Transcriptomic Comparison of Common Nontyphoidal Salmonella Serovars Provides Greater Resolution for Understanding Differences in Virulence

RNA extracted from representative isolates of each serovar grown to late exponential phase in Luria-Bertani broth showed that transcript abundances of core genes were significantly higher (p < 0.05) than those of accessory genes for all three serovars. Inter-serovar comparisons demonstrated higher transcript abundances for genes related to vitamin B12 biosynthesis, and ethanolamine and 1,2-propanediol utilization in S. Cerro compared to Javiana and Typhimurium, while genes in Salmonella Pathogenicity Island (SPI) 1 had significantly higher transcript abundances in S. Javiana and S. Typhimurium. Overall design: mRNA profiles from three Salmonella serovars (Cerro, Javiana, Typhimurium) were generated by deep sequencing with an Illumina NextSeq 500 in triplicate with 75-nt stranded single-end reads.