Gene expression in small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) cell line COV434. Gene expression in small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) cell line COV434

Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare and aggressive form of ovarian cancer. SCCOHT tumors have inactivating mutations in SMARCA4 (BRG1), one of the two mutually exclusive ATPases of the SWI/SNF chromatin remodeling complex. To address the role that SMARCA4 loss plays in SCCOHT tumorigenesis, we performed RNA-seq in a SCCOHT cell line +/- SMARCA4 re-expression. SMARCA4 re-expression was acheived by integration of pIND20-BRG1 and inducing with doxycycline. The goal of this study was to generate a high sequencing depth RNA-seq dataset to determine how restoration of SMARCA4 results in changes in gene expression and RNA splicing. Overall design: COV434 pIND20 BRG1-2.1 and parental COV434 cell lines were induced as previously described (PMID:29440177) with and without doxycycline to re-express SMARCA4. Experiment was repeated in independent triplicate.