Smart-Seq of male blastocysts from mothers bearing the OgtT931A mutation.. Smart-Seq of male blastocysts from mothers bearing the OgtT931A mutation.

40-bp PE mRNA-Seq of single E4 blastocysts generated through IVF of oocytes harvested from two F0 OgtT931A/WT females and from two littermate WT females, using the same WT sperm. Sex was established by PCR genotyping of the cDNA and only male blastocysts were sequenced. One sample (het_1A) is a female (deduced in silico from the sequencing data) but it is present because of incorrect PCR genotyping. Presence of the mutation among the embryos from the OgtT931A/WT females was also enstablished from the cDNA via PCR genotyping and Sanger sequencing - only two hemizygous OgtT931A/Y embryos were found (het_3D, het_5G).