MicroRNA Expression Profiling of Lumbar Spinal Cord in a Rat Model of Bone Cancer Pain

Bone cancer pain (BCP), in which the majority is caused by metastasis from other cancer sites, is the most common type of chronic cancer pain. Bone cancer pain is developed accompanied by changes of numerous genes expression, from peripheral to central nervous system, which may account for this dysfunctional nociceptive perception. Although the pivotal role of lncRNA in neuropathic pain are well acknowledged, the involvement of lncRNA in bone cancer pain development are remain to be clarified. Thus, in the present study we performed transcriptome sequencing to explore changes in expression profiles of lncRNA and mRNA to provide a landscape of dysregulated miRNA in spinal cord of bone cancer pain. Overall design: On the fourteenth day after tumor inoculation, total RNA was extracted from the ipsilateral side of spinal cord of bone-cancer-pained and sham-operated Wistar rats, in triplicates, and high throughput sequencing of ncRNA and mRNA was performed by Illumina NovaSeq 6000.