Expression analysis of knockdown of USP7 in HEK293T cells.

Ubiquitin Specific Peptidase 7 (USP7) is a deubiquitinase of several important regulatory proteins, and important regulator of TGFb pathway. To investigate their role in cell signaling, we analyzed global mRNA levels in HEK293T cells that were knocked down with shRNAs against USP7 and non-targeting control (CTRL). HEK293T cells were transfected with knockdown shRNA plasmids to reduce protein levels of USP7, or none (control knockdown with non-targeting shRNA plasmid). After 78h of knockdown, we isolated total RNA. We pairwise compared mRNA levels from three control samples to three USP7 knockdown samples, six samples total.