Structural cryo-electron microscopy data store
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Cryo-electron microscopy (cryo-EM) is a vital structural biology tool that enables the determination of three-dimensional structures of large biological macromolecules, macromolecular complexes, and cell components of biomedical interest, which bridges the gap of complementary structural biology tools such as X-ray crystallography and nuclear magnetic resonance spectroscopy (NMR). The resolution of single-particle cryo-EM has dramatically improved in the last couple of years and cryo-EM is now an essential technique for structural biology research. When operating in "molecular movie mode" the Titan Krios cryo-EM with Falcon II direct electron detector will produce major data volumes, generating terabytes of images per day. The data produced will be a significant asset to Australian and international researchers and will permit the determination of structures of large complex proteins that resist characterization through approaches such as X-ray crystallography. The data storage made available through this proposal will allow researchers to more easily capture, share and publish images produced using the high-end microscopes of the Clive and Vera Ramaciotti Centre for Structural Cryo-Electron Microscopy. More broadly, this undertaking is important to support Australian investment in characterization, providing the missing fabric to collect, collate, and collaborate over data. It will form the basis for a sophisticated workflow, developed under NeCTAR’s Characterisation Virtual Laboratory, that includes significant Australian research infrastructure like the NeCTAR Cloud, MASSIVE, RDSI and VicNode. This Collection will make imaging data from research grants totalling over $100M and instrument grants totalling more than $7M accessible to researchers and provide a mechanism to make the primary research data underlying published research broadly available. This includes data produced by the TitanTM Krios cryo TEM at the Clive and Vera Ramaciotti Centre for Structural Cryo-Electron Microscopy (Monash, in collaboration with WEHI, La Trobe University, the University of Melbourne, Burnet Institute and the Peter MacCallum Cancer Centre). This collection will underpin the $27.9M ARC Centre of Excellence in Advanced Molecular Imaging, which includes collaborators from Monash University, UNSW, UQ, The University of Melbourne, La Trobe University, Australian Synchrotron, University of Warwick, ANSTO, Deutsches Elektronen-Synchrotron, Leica Microsystems Pty Ltd and Carl Zeiss Pty Ltd.
冷冻电子显微镜（Cryo-electron microscopy, cryo-EM）是结构生物学领域不可或缺的研究工具，可解析具有生物医学研究价值的大型生物大分子、大分子复合物及细胞组分的三维结构，填补了X射线晶体学、核磁共振波谱法（NMR）等互补结构生物学工具的技术空白。近年来，单颗粒冷冻电镜的分辨率实现了跨越式提升，如今已成为结构生物学研究的核心技术。当以“分子电影模式”运行时，搭载Falcon II直接电子探测器的Titan Krios冷冻电子显微镜将产生海量数据，单日可生成太字节级别的图像数据。此类数据将成为澳大利亚及全球研究者的重要科研资产，助力解析那些难以通过X射线晶体学等手段完成表征的大型复杂蛋白质结构。 本科研项目获批的数据存储设施，将使研究者能够更便捷地获取、共享与发布克莱夫与维拉·拉马乔蒂结构冷冻电子显微镜中心的高端显微镜所产生的成像数据。从更宏观的层面来看，该举措对于支撑澳大利亚在生物表征领域的科研投入至关重要，可为数据的收集、整理与协作研究填补关键环节。它将成为依托NeCTAR表征虚拟实验室开发的先进工作流的核心基础，该工作流涵盖NeCTAR云、MASSIVE超算中心、RDSI及VicNode等澳大利亚重要科研基础设施。 本数据集将面向研究者开放来自总价值超1亿澳元的研究项目及总价值超700万澳元的仪器购置拨款的成像数据，并建立配套机制，使已发表研究依托的原始科研数据得以广泛共享。这些数据包括克莱夫与维拉·拉马乔蒂结构冷冻电子显微镜中心（莫纳什大学，合作方包括WEHI、拉筹伯大学、墨尔本大学、伯纳特研究所及彼得·麦卡勒姆癌症中心）的Titan™ Krios冷冻透射电子显微镜所产生的数据。本数据集将为总投入达2790万澳元的澳大利亚研究委员会（Australian Research Council, ARC）高级分子成像卓越中心提供核心支撑，该中心的合作方包括莫纳什大学、新南威尔士大学（UNSW）、昆士兰大学（UQ）、墨尔本大学、拉筹伯大学、澳大利亚同步加速器、华威大学、澳大利亚核科学技术组织（ANSTO）、德国电子同步加速器（Deutsches Elektronen-Synchrotron）、徕卡显微系统私人有限公司及卡尔·蔡司私人有限公司。
