How groundwater abstraction impacts groundwater ecosystems

Groundwater pump test - see Methods -- Read me.txt file for data collection methods.

Microbial assemblage data derived from Illumina sequencing of 16S rDNA amplicons. Sequence data were processed using Greenfield Hybrid Analysis Pipeline (GHAP) v2.2, created by CSIRO Australia (available at https://cloudstor.aarnet.edu.au/plus/s/ pUvwME6fMeCcX - zL). Taxonomic assignment is based on the Ribosomal Database Project (RDP) Classifier (Wang, Garrity et al. 2007). OTUs were assigned using a 97% similarity threshold). Low abundant OTUs (<10), organelles and unassigned taxa at kingdom level were removed.